ECLIPSE Kick-off Meeting

ECLIPSE Kick-off Meeting was held in Bologna (Italy) last 3-4 May 2022, hosted by the University of Bologna in the charming and recently restored Palazzo Malvezzi (Sala Armi and Sala Feste).

The Kick-Off Meeting brought together all members of the consortium and it has provided an opportunity for an update on the main objective of the project and the launching of initial actions.

The key point have been set, the Consortium is ready to achieve the project goals.

Luisa De Cola and Luca Prodi will attend the EuChemS2022 Conference

Luisa De Cola and Luca Prodi (lecture IL.G2.1) will attend the EuChemS2022 Conference in Lisbon  (August 28-September 1)

About the event

Luca Prodi will attend at the 5th International Caparica Conference on Chromogenic and Emissive Materials 2022

Luca Prodi will attend at the 5th International Caparica Conference on Chromogenic and Emissive Materials 2022; Caparica, Portugal, July 4-8, giving a lecture on “Silica Nanoparticles as Photoactive Organized Systems for Nanomedicine”

About the event

Advanced photodynamic therapy with an engineered M13 phage targeting EGFR

Photodynamic therapy (PDT) is a potential synergistic approach to chemotherapy for treating ovarian cancer, the most lethal gynecologic malignancy. Here we used M13 bacteriophage as a targeted vector for the efficient photodynamic killing of SKOV3 and COV362 cells. The M13 phage was refactored (M13r) to display an EGFR binding peptide in its tip that is frequently overexpressed in ovarian cancer. The refactored phage was conjugated with chlorin e6 (Ce6), one of the most widely used photosensitizers (M13r-Ce6). The new platform, upon irradiation, generated ROS by type I mechanism and showed activity in killing SKOV3 and COV362 cells even at concentrations in which Ce6 alone was ineffective. A microscopy analysis demonstrated an enhanced cellular uptake of M13r-Ce6 compared to free Ce6 and its mitochondrial localization. Western blot analysis revealed significant downregulation in the expression of EGFR in cells exposed to M13r-Ce6 after PDT. Following PDT treatment, autophagy induction was supported by an increased expression of LC3II, along with a raised autophagic fluorescent signal, as observed by fluorescence microscopy analysis for autophagosome visualization. As a conclusion we have herein proposed a bacteriophage-based receptor targeted photodynamic therapy for EGFR-positive ovarian cancer.

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Publication by Sabrina Conoci, Luca Prodi and Giovanni Valenti on Biosensors and Bioelectronics

P. Nikolaou, E. L. Sciuto, A. Zanut, S. Petralia, G. Valenti, F. Paolucci, L. Prodi, S. Conoci, “Ultrasensitive PCR-Free Detection of Whole Virus Genome by Electrochemiluminescence”, Biosensors and Bioelectronics, 209, 114165 (2022).

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